<i>In situ</i> EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code
Anandi Kugele, Sophie Ketter, Bjarne Silkenath, Valentin Wittmann, Benesh Joseph, Malte Drescher
Abstract
Diels-Alder click chemistry of the genetically encoded amino acid SCO-L-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance measurements, and the temporally shifted activation of the nitroxide allows for advanced experimental setups. This study describes significant evolution of Diels-Alder-mediated spin labelling on cellular surfaces and opens up new vistas for the the study of membrane proteins.
Topics & Concepts
Nitroxide mediated radical polymerizationElectron paramagnetic resonanceSite-directed spin labelingMembraneTransporterEscherichia coliGenetic codeChemistryLysineAmino acidBiophysicsIn situLabellingSpin labelBiochemistryClick chemistryPolymerBiologyCombinatorial chemistryNuclear magnetic resonanceGeneOrganic chemistryPhysicsCopolymerRadical polymerizationElectron Spin Resonance StudiesLanthanide and Transition Metal ComplexesAnalytical Chemistry and Sensors