High-throughput force measurement of individual kinesin-1 motors during multi-motor transport
Saurabh Shukla, Alice Troitskaia, Nikhila Swarna, Barun Kumar Maity, Marco Tjioe, Carol S. Bookwalter, Kathleen M. Trybus, Yann R. Chemla, Paul R. Selvin
Abstract
. We find that kinesins primarily exerted less than 1 pN force, even while the microtubule is bypassing artificial obstacles of 20-100 nanometer size. Occasionally, individual forces increase upon encountering obstacles, although at other times they do not, with the cargo continuing in a directional manner. Our high-throughput technique, which can measure forces by many motors simultaneously, is expected to be useful for many different types of molecular motors.
Topics & Concepts
KinesinThroughputMolecular motorMeasure (data warehouse)MicrotubuleMotor proteinNanotechnologyPhysicsComputer scienceMaterials scienceBiologyTelecommunicationsCell biologyDatabaseWirelessForce Microscopy Techniques and ApplicationsAdvanced biosensing and bioanalysis techniquesMolecular Junctions and Nanostructures