Principles and Problems of Exosome Isolation from Biological Fluids
Edward Yakubovich, A. G. Polischouk, В И Евтушенко
2022Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology150 citationsDOIOpen Access PDF
Abstract
Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic and therapeutic potential, but the lack of standardized methods for their efficient isolation and analysis limits the introduction of exosomal technologies into clinical practice. This review discusses the problems associated with the isolation of exosomes from biological fluids, as well as the principles of traditional and alternative methods of isolation. The aim of the presented review is to illustrate the variety of approaches based on the physical and biochemical properties of exosomes that can be used for exosome isolation. The advantages and disadvantages of different methods are discussed.
Topics & Concepts
MicrovesiclesIsolation (microbiology)ExosomeExtracellular vesiclesComputational biologyBiological fluidsBiologyChemistryBioinformaticsCell biologymicroRNABiochemistryChromatographyGeneExtracellular vesicles in diseaseRNA Interference and Gene DeliveryMicroRNA in disease regulation