Transcriptome Analyses of Near Isogenic Lines Reveal Putative Drought Tolerance Controlling Genes in Wheat
Sina Nouraei, Md Sultan Mia, Hui Liu, Neil C. Turner, Guijun Yan
Abstract
Graphical Abstract The workflow of this study. (1) Near isogenic lines for qDSI.4B.1 QTL responsible for drought tolerance were grown in control and drought stress conditions, (2) grain samples were collected at 7 and 14 days after stress initiation at anthesis, (3) RNA was extracted, (4) sequenced and (5) data were analysed and organized with focus on 4BS, (6) six candidate genes were found for drought tolerance in qDSI.4B.1 interval and sequencing results were confirmed by qRT-PCR, (7) the protein products and (8) molecular function of the candidate genes were future studied.
Topics & Concepts
BiologyCandidate geneGeneQuantitative trait locusGeneticsDrought toleranceLocus (genetics)TranscriptomeSingle-nucleotide polymorphismGene expressionBotanyGenotypeWheat and Barley Genetics and PathologyGenetics and Plant BreedingGenetic Mapping and Diversity in Plants and Animals