Heterologous Gene Expression System Using the Cold-Inducible <i>CnAFP</i> Promoter in <i>Chlamydomonas reinhardtii</i>
Minjae Kim, Jongrae Kim, Sanghee Kim, EonSeon Jin
Abstract
was shortened to 600 bp, indicating the presence of a low-temperature response element between -600 and -477 bp. Our results show the availability of new heterologous gene expression systems with cold-inducible promoters and the possibility to find novel low-temperature response factors in microalgae. Through further improvement, this cold-inducible promoter could be used to develop more efficient expression tools.
Topics & Concepts
Chlamydomonas reinhardtiiPromoterBiologyGene expressionReporter geneGeneHeterologousChlamydomonasLuciferaseMolecular biologyHeterologous expressionExpression vectorRegulation of gene expressionGeneticsRecombinant DNATransfectionMutantAlgal biology and biofuel productionPhysiological and biochemical adaptationsAquaculture Nutrition and Growth