Gan Shen Fu Fang ameliorates liver fibrosis <i>in vitro</i> and <i>in vivo</i> by inhibiting the inflammatory response and extracellular signal-regulated kinase phosphorylation
Qinghong Du, Chu-Jun Zhang, Weihong Li, Yan Mu, Ya Xu, Scott Lowe, Lin Han, Xue Yu, Shuyan Wang, Yu Li, Jian Li
Abstract
BACKGROUND: in an attempt to provide some hope for the treatment. AIM: and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase (ERK) phosphorylation. METHODS: experiments. Hematoxylin and eosin staining and Masson staining, biochemical assays, hydroxyproline (Hyp) assays, enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis, liver function, the inflammatory response and ERK phosphorylation. The CCK8 assay, immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells. RESULTS: results, GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB. CONCLUSION: . These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.