Excitation ratiometric chloride sensing in a standalone yellow fluorescent protein is powered by the interplay between proton transfer and conformational reorganization
Cheng Chen, Jasmine N. Tutol, Longteng Tang, Liangdong Zhu, Whitney S. Y. Ong, Sheel C. Dodani, Chong Fang
Abstract
but turn-off response to other anions such as nitrate as governed by different binding affinities. These deep mechanistic insights lay the foundation for guiding the targeted engineering of phiYFP and its derivatives for ratiometric imaging of cellular chloride with high selectivity.
Topics & Concepts
ChromophoreFluorescencePhotochemistryChemistryUltrafast laser spectroscopyExcited stateGreen fluorescent proteinStackingAbsorption (acoustics)SpectroscopyMaterials scienceGeneBiochemistryComposite materialQuantum mechanicsNuclear physicsOrganic chemistryPhysicsPhotoreceptor and optogenetics researchAdvanced Fluorescence Microscopy Techniquesbioluminescence and chemiluminescence research