Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice
Ryotaro Kano, T. Kusano, R Takeda, Hideki Shirakawa, David C. Poole, Yutaka Kano, Daisuke Hoshino
Abstract
This in vivo model successfully characterized the effects of eccentric (ECC) and concentric (CONC) contractions on cytosolic and mitochondrial [H 2 O 2 ] in mouse skeletal muscle. Compared with CONC, ECC induced higher and more sustained [H 2 O 2 ] cyto —an effect that was abolished by Nox2 inhibition. ECC-induced [H 2 O 2 ] cyto elevations were requisite for altered gene expression.
Topics & Concepts
Skeletal muscleContraction (grammar)EccentricNADPH oxidaseTibialis anterior muscleEndocrinologyInternal medicineMuscle contractionBiologyChemistryGene expressionAnatomyGeneBiochemistryOxidative stressMedicineQuantum mechanicsPhysicsExercise and Physiological ResponsesMitochondrial Function and PathologyCardiovascular and exercise physiology