Assessment of cancer cell migration using a viscosity-sensitive fluorescent probe
Xiaohong Pan, Cheng Wang, Congcong Zhao, Tingting Cheng, Aishan Zheng, Yuru Cao, Kehua Xu
Abstract
A novel viscosity probe (NV1) was developed for assessing cancer cell migration. NV1 can respond to changes of viscosity rapidly and exhibits high sensitivity in HepG2 cells treated with starvation, rotenone and nystatin. Importantly, NV1 was used for the first time to evaluate the relationship between intracellular viscosity changes and cancer cell migration and proved that increased intracellular viscosity inhibits cell migration while decreased intracellular viscosity promotes cell migration.
Topics & Concepts
IntracellularViscosityCell migrationCellChemistryBiophysicsCancer cellFluorescenceCancerBiochemistryMaterials scienceBiologyMedicineInternal medicineComposite materialQuantum mechanicsPhysicsNanoparticle-Based Drug DeliveryLipid Membrane Structure and BehaviorMass Spectrometry Techniques and Applications