Improving the soluble expression of difficult-to-express proteins in prokaryotic expression system via protein engineering and synthetic biology strategies
Jin‐Ping Chen, Jin‐Song Gong, Chang Su, Heng Li, Zhenghong Xu, Jin‐Song Shi
Topics & Concepts
BiologyProtein expressionComputational biologyProtein engineeringSynthetic biologyProtein foldingDirected evolutionCRISPRCas9BiochemistryGeneEnzymeMutantCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in InsectsRNA and protein synthesis mechanisms