Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes
Chellapandian Balachandran, Alagumalai Vishali, Natarajan Arun Nagendran, Kathirvelu Baskar, Abeer Hashem, Elsayed Fathi Abd Allah
Abstract
Marine microbes are potential source for novel metabolites. They are efficient in producing these metabolites utilizing agrowastes. Protease is one of the enzymes which find wide industrial applications. In the present study, protease producing bacteria was isolated from marine sediments and the organism was identified as Bacillus halodurans. The organism was subjected to protease production under solid state fermentation (SSF) using different agrowastes as substrates. Among the substrates used, wheat bran yielded maximum quantity of protease. The fermentation process was carried out under different cultural conditions to optimize the parameters influencing the enzyme production. The results of the stain removal studies by the enzyme revealed the increased efficiency of the microbial enzyme than the commercial detergent.