Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production
Songyuan Li, Christian Bille Jendresen, Jenny Landberg, Lasse Ebdrup Pedersen, Nikolaus Sonnenschein, Sheila Ingemann Jensen, Alex Toftgaard Nielsen
Abstract
shows more than 5-fold increase in GFP accumulation and 45% decrease in biomass formation. Overall, our genome-wide CRISPRi screen provides key targets for growth decoupling, and the approach can be applied to improve biobased production in other microorganisms.
Topics & Concepts
Decoupling (probability)GenomeComputational biologyBiologySynthetic biologyEscherichia coliGenome editingBiotechnologyBiochemical engineeringGeneGeneticsEngineeringControl engineeringCRISPR and Genetic EngineeringMicrobial Metabolic Engineering and BioproductionViral Infectious Diseases and Gene Expression in Insects