Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins
Ramona Birke, Julia Ast, Dorien A. Roosen, Joon Lee, Kilian Roßmann, Christiane Huhn, Bettina Mathes, Michael Lisurek, David Bushiri, Han Sun, Ben Jones, Martin Lehmann, Joshua Levitz, Volker Haucke, David J. Hodson, Johannes Broichhagen
Abstract
self-labelling protein tags. We anticipate that Sulfo549, Sulfo646 and their congeners will be useful for a number of cell biology applications where labelling of intracellular sites interferes with accurate surface protein analysis.
Topics & Concepts
HaloSTED microscopyRed CellFar-redNile redRhodamineChemistryFluorescenceSuperresolutionComputer sciencePhysicsOpticsRed lightBiologyArtificial intelligenceAstronomyGalaxyImage (mathematics)BotanyMonoclonal and Polyclonal Antibodies ResearchBiotin and Related StudiesClick Chemistry and Applications