Nucleoside analogs in ADAR guide strands targeting 5′-UA sites
Hannah F. Brinkman, Victorio Jauregui Matos, Herra G. Mendoza, Erin Doherty, Peter A. Beal
Abstract
W104X site expressed on a reporter transcript in human cells. This work demonstrates the importance of the -1 position of the gRNA to ADAR editing and discloses nucleoside analogs for this site that modulate ADAR editing efficiency.
Topics & Concepts
ADARNucleosideChemistryPharmacologyComputational biologyStereochemistryBiologyBiochemistryRNA editingRNAGeneRNA regulation and diseaseRNA and protein synthesis mechanismsRNA Interference and Gene Delivery