Revisiting PFA-mediated tissue fixation chemistry: FixEL enables trapping of small molecules in the brain to visualize their distribution changes
Hiroshi Nonaka, Takeharu Mino, Seiji Sakamoto, Jae Hoon Oh, Yu Watanabe, Mamoru Ishikawa, Akihiro Tsushima, Kazuma Amaike, Shigeki Kiyonaka, Tomonori Tamura, A.R. Aricescu, Wataru Kakegawa, Eriko Miura, Michisuke Yuzaki, Itaru Hamachi
Abstract
is used to obtain images of the distribution of the small molecules, which addresses selective/nonselective binding to proteins, time-dependent localization changes, and diffusion/retention kinetics of MOIs such as the scaffold of PET tracer derivatives or drug-like small molecules.
Topics & Concepts
Small moleculeChemistryKineticsMoleculeFixation (population genetics)BiophysicsParaformaldehydeDrug deliveryNanotechnologyBiochemistryBiologyMaterials scienceOrganic chemistryPhysicsQuantum mechanicsGeneAdvanced Fluorescence Microscopy TechniquesMedical Imaging Techniques and ApplicationsRadiopharmaceutical Chemistry and Applications