Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells
Fu‐Shi Quan, Gi‐Ja Lee
Abstract
Hydrogen sulfide (H 2 S) plays an important role in mammals as a signaling molecule. Recently, abnormal H 2 S concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer’s disease, and Parkinson’s disease. As regulating H 2 S concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to H 2 S research. To understand the role of H 2 S in pathophysiology and develop H 2 S‐based therapies, it is necessary to measure the exact concentration of H 2 S within biological systems. But, H 2 S is volatile and can be easily oxidized. Besides, the active sites for several biological effects of H 2 S are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of H 2 S within live cells. This review provides a summary of recent developments in H 2 S detection methods for live cell analysis.