Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry
Jessica L. Nickerson, Alan A. Doucette
Abstract
. Furthermore, analysis of the protein pellet by bottom-up mass spectrometry (MS) reveals unbiased recovery of all proteins with respect to molecular weight, isoelectric point (pI), and hydrophobicity. Our robust strategy to isolate proteins maximizes recovery and throughput, exploiting the analytical advantages of precipitation over alternative techniques. Data are available via ProteomeXchange with identifier PXD015674.
Topics & Concepts
ProteomeProtein precipitationMass spectrometryChemistryChromatographyIsoelectric pointPrecipitationAcetoneProteomicsTandem mass spectrometryIsoelectric focusingBiochemistryEnzymeGenePhysicsMeteorologyAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies