Extracellular Vesicle Formation in Cryptococcus deuterogattii Impacts Fungal Virulence and Requires the <i>NOP16</i> Gene
Rafael F. Castelli, Alana Kelyene Pereira, Leandro Honorato, Alessandro F. Valdez, Haroldo César de Oliveira, Jaqueline Moraes Bazioli, Ane Wichine Acosta Garcia, Tabata D’Maiella Freitas Klimeck, Flavia C. G. Reis, Charley Christian Staats, Leonardo Nimrichter, Taícia Pacheco Fill, Márcio L. Rodrigues
Abstract
Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii . In this study, during the phenotypic characterization of C. deuterogattii mutants lacking NOP16 expression, we observed that this gene was required for EV production.
Topics & Concepts
BiologyVirulenceExtracellularMutantExtracellular vesiclesGeneExtracellular vesicleCryptococcus neoformansMicrobiologyCryptococcusGene expressionVesicleCell biologyPhenotypeVirulence factorFungal proteinMicrovesiclesGeneticsMembranemicroRNAFungal Infections and StudiesExtracellular vesicles in diseasePhytoplasmas and Hemiptera pathogens