Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence
Elizabeth K. Neumann, Nathan Heath Patterson, Jamie Allen, Lukasz G. Migas, Haichun Yang, Maya Brewer, David M. Anderson, Jennifer Harvey, Danielle Gutierrez, Raymond C. Harris, Mark deCaestecker, Agnes B. Fogo, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Abstract
Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.