Efficient Light‐Harvesting Antennae Resulting from the Dense Organization of Dyes into DNA Junctions through <scp>d</scp>‐Threoninol
Hiromu Kashida, Hidenori Azuma, Ryoko Maruyama, Yasuyuki Araki, Takehiko Wada, Hiroyuki Asanuma
Abstract
Herein we report the construction of efficient light-harvesting antennae by hybridization of DNA oligonucleotides containing high densities of fluorophores into DNA junctions through d-threoninol. Six pyrene donors could be incorporated into each arm without self-quenching. A perylene acceptor was located at the center of the junction. Antenna effects of a duplex and three- to eight-way junctions were systematically compared. Six- and eight-way junctions had the highest antenna effects, and their effective absorption coefficients were 8.5 times higher than that of perylene. Interestingly, even-numbered junctions had higher efficiencies than odd-numbered junctions. Nondenaturing gel analyses and fluorescence lifetime measurements demonstrated that the strong odd-even effects were derived from differences in the stability of junctions. The results presented will guide the design of efficient artificial photosynthetic systems.