Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and Mucormycosis
Sunil Kumar, Ayaan Ahmad, Namrata Kushwaha, Niti Shokeen, Sheetal Negi, Kamini Gautam, Anup Singh, Pavan Tiwari, Rakesh Garg, Richa Agarwal, Anant Mohan, Anjan Trikha, Alok Thakar, Vikram Saini
Abstract
, the most commonly used reference gene in COVID-19 studies, turned out to be the least suitable. This work fills an important gap in the field and promises to facilitate determination of an accurate expression of genes to catalyze development of novel molecular diagnostics and therapeutics for improved patient care.
Topics & Concepts
Reference genesBiologyHousekeeping geneGeneGene expressionGlyceraldehyde 3-phosphate dehydrogenaseReal-time polymerase chain reactionMolecular biologyGeneticsMolecular Biology Techniques and ApplicationsOrthopedic Infections and TreatmentsVirus-based gene therapy research