<i>In vivo</i> delivery of plasmid DNA by lipid nanoparticles: the influence of ionizable cationic lipids on organ-selective gene expression
Azizah Algarni, Emily H. Pilkington, Estelle J. A. Suys, Hareth Al-Wassiti, Colin W. Pouton, Nghia P. Truong
Abstract
protein production in the spleen than in the liver. This work sheds light on the importance of the choice of ionizable cationic lipid and nucleic acid cargo for organ-selective gene expression. The study also provides a new design principle towards the formulation of more effective LNPs for biomedical applications of pDNA, such as gene editing, vaccines and immunotherapies.
Topics & Concepts
Gene deliveryTransfectionChemistryIn vivoCationic polymerizationNucleic acidDNARNA interferenceGenetic enhancementLiposomeCell biologyBiophysicsGeneRNABiochemistryBiologyGeneticsOrganic chemistryRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesAnimal Virus Infections Studies