Unraveling the Photoactivation Mechanism of a Light-Activated Adenylyl Cyclase Using Ultrafast Spectroscopy Coupled with Unnatural Amino Acid Mutagenesis
Jinnette Tolentino Collado, James N. Iuliano, Katalin Pirisi, Samruddhi S. Jewlikar, Katrin Adamczyk, Gregory M. Greetham, Michael Towrie, Jeremy R. H. Tame, Stephen R. Meech, Peter J. Tonge, András Lukács
Abstract
. Our results thus show that proton transfer plays an essential role in initiating the structural reorganization of the AC domain that results in AC activity.
Topics & Concepts
Flavin groupChemistryFlavoproteinAdenylyl cyclaseFlavin adenine dinucleotidePhotochemistryProtonationFlavin mononucleotideStereochemistryEnzymeBiochemistryCofactorOrganic chemistryIonPhotoreceptor and optogenetics researchbioluminescence and chemiluminescence researchLight effects on plants