Immune Response to Conjugates of Fragments of the Type K9 Capsular Polysaccharide of Acinetobacter baumannii with Carrier Proteins
Н. В. Руденко, A. P. Karatovskaya, A. V. Zamyatina, A. O. Shepelyakovskaya, С. Г. Семушина, Ф. А. Бровко, Anna M. Shpirt, В. И. Торгов, Natalia G. Kolotyrkina, Alexandr Zinin, Anastasiya A. Kasimova, Andrei V. Perepelov, Mikhail M. Shneider, Yuriy A. Knirel
Abstract
Immunization by glycoconjugates with A. baumannii type K9 CPS fragments induced a high level of antibodies (predominantly IgG) in sera, which reacted specifically with the CPS of A. baumannii type K9, as well as a long immunological memory. The sera of immunized animals efficiently opsonized A. baumannii type K9. Immunization resulted in the balanced production of pro/anti-inflammatory lymphokines and protective antibodies to ensure the survival of the mice infected with A. baumannii. The level of specific antibodies was sufficient to provide protective immunity against the challenge by A. baumannii, making this approach applicable in the development of vaccine preparations.