Effective Multi-lineage Engraftment in a Mouse Model of Fanconi Anemia Using Non-genotoxic Antibody-Based Conditioning
Meera Srikanthan, Olivier Humbert, Kevin G. Haworth, Christina Ironside, Yogendra Singh Rajawat, Bruce R. Blazar, Rahul Palchaudhuri, Anthony E. Boitano, M. Cooke, David T. Scadden, Hans‐Peter Kiem
Abstract
knockout mice were conditioned with either CD45-SAP or CD117-SAP prior to receiving whole marrow from a heterozygous healthy donor. Bone marrow and peripheral blood analysis revealed equivalent levels of donor engraftment, with minimal toxicity in ADC-treated groups as compared with cyclophosphamide-treated controls. Our findings suggest ADCs may be an effective conditioning strategy in stem cell transplantation not only for diseases where traditional chemotherapy is not tolerated, but also more broadly for the field of blood and marrow transplantation.