Exploring the potential therapeutic effect of <i>Eucommia ulmoides</i>–<i>Dipsaci Radix</i> herbal pair on osteoporosis based on network pharmacology and molecular docking technology
Shuai Feng, Ting Wang, Liming Fan, Xinxin An, Xinli Ding, Minjuan Wang, Xifeng Zhai, Yanjun Cao, Jiao He, Li Yang
Abstract
-value < 0.05. Sybyl-x 2.1.1 and Autodock Vina 1.1.2 software were used to cross validate the affinity between active compounds and target proteins. Our results showed that a total of 50 active compounds were screened, corresponding to 895 EU-DR targets, 2202 OP targets and 144 shared targets. The flavonoids in EU-DR played an important role in anti-OP. The enrichment analysis of GO and KEGG suggested EU-DR exerted a therapeutic effect on OP mainly by regulating the osteoclast differentiation related signaling pathway. Meanwhile, molecular docking results showed that most active compounds in EU-DR had strong binding efficiency to the target proteins. In conclusion, this study elaborated the multi-component, multi-target, and multi-pathway interaction mechanism of the EU-DR herbal pair in the treatment of OP for the first time, which also provided a pharmacological basis for treating OP.