Exploring the entropy-driven amplification reaction and <i>trans</i>-cleavage activity of CRISPR-Cas12a for the development of an electrochemiluminescence biosensor for the detection of the SARS-CoV-2 RdRp gene in real samples and environmental surveillance
Kai Zhang, Zhenqiang Fan, Yuedi Ding, Sha Zhu, Minhao Xie, Nan Hao
Abstract
An ECL biosensor based on entropy-driven amplification and CRISPR-Cas12a shear for the detection of the SARS-CoV-2 RdRp gene in environmental surveillance was reported.
Topics & Concepts
CRISPRBiosensorElectrochemiluminescenceGeneGuide RNAComputational biologyChemistryBiologyMolecular biologyCas9GeneticsDetection limitBiochemistryChromatographyAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringSARS-CoV-2 and COVID-19 Research