Epigenetic silencing <i>ZSCAN23</i> promotes pancreatic cancer growth by activating Wnt signaling
Qian Du, Meiying Zhang, Aiai Gao, Tao He, Mingzhou Guo
Abstract
Pancreatic ductal adenocarcinoma (PDAC) is the most malignant tumor.Zinc finger and SCAN domaincontaining protein 23 (ZSCAN23) is a new member of the SCAN domain family.The expression regulation and biological function remain to be elucidated.In this study, we explored the epigenetic regulation and the function of ZSCAN23 in PDAC.ZSCAN23 was methylated in 60.21% (171/284) of PDAC and its expression was regulated by promoter region methylation.The expression of ZSCAN23 inhibited cell proliferation, colony formation, migration, invasion, and induced apoptosis and G1/S phase arrest.ZSCAN23 suppressed Panc10.05cell xenograft growth in mice.Mechanistically, ZSCAN23 inhibited Wnt signaling by interacting with myosin heavy chain 9 (MYH9) in pancreatic cancer cells.ZSCAN23 is frequently methylated in PDAC and may serve as a detective marker.ZSCAN23 suppresses PDAC cell growth both in vitro and in vivo.