Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells
Kimberly R. Long, Youssef Rbaibi, Corry D. Bondi, B. Rhodes Ford, Amanda C. Poholek, Cary R. Boyd‐Shiwarski, Roderick J. Tan, Joseph Locker, Ora A. Weisz
Abstract
Megalin and cubilin receptors together with their adaptor protein Dab2 represent major components of the endocytic machinery responsible for efficient uptake of filtered proteins by the proximal tubule (PT). Dab2 and megalin expression have been implicated as both positive and negative modulators of kidney disease. We used RNA sequencing to knock out CRISPR/Cas9 cubilin, megalin, and Dab2 in highly differentiated PT cells to identify PT-specific changes that are directly consequent to knockout of each component.
Topics & Concepts
CRISPRProximal tubuleKnockout mouseCell biologyTranscription (linguistics)KidneyChemistryTranscription factorBiologyMolecular biologyGeneGeneticsBiochemistryLinguisticsPhilosophyRenal and related cancersGenetic and Kidney Cyst DiseasesPluripotent Stem Cells Research