Neuronal wiring diagram of an adult brain
Sven Dorkenwald, Arie Matsliah, Amy Sterling, Philipp Schlegel, Szi-chieh Yu, Claire McKellar, Albert Lin, Marta Costa, Katharina Eichler, Yijie Yin, Will Silversmith, Casey M Schneider-Mizell, Chris S. Jordan, Derrick Brittain, Akhilesh Halageri, Kai Kuehner, Oluwaseun Ogedengbe, R. A. Morey, Jay Gager, Krzysztof Kruk, Eric Perlman, Runzhe Yang, David Deutsch, Doug Bland, Marissa Sorek, Ran Lu, Thomas Macrina, Kisuk Lee, J. Alexander Bae, Shang Mu, Barak Nehoran, Eric Mitchell, Sergiy Popovych, Jingpeng Wu, Zhen Jia, Manuel Castro, Nico Kemnitz, Dodam Ih, Alexander Shakeel Bates, Nils Eckstein, Jan Funke, Forrest Collman, Davi D. Bock, Gregory S.X.E. Jefferis, H. Sebastian Seung, Mala Murthy, Zairene Lenizo, Austin T. Burke, Kyle Patrick Willie, Nikitas Serafetinidis, Nashra Hadjerol, Ryan Willie, Ben Silverman, John Anthony Ocho, Joshua Bañez, Rey Adrian Candilada, Anne Kristiansen, Nelsie Panes, Arti Yadav, Remer Tancontian, Shirleyjoy Serona, Jet Ivan Dolorosa, Kendrick Joules Vinson, Dustin Garner, Regine Salem, Ariel Dagohoy, Jaime Skelton, Mendell Lopez, Laia Serratosa Capdevila, Griffin Badalamente, Thomas Stocks, Anjali Pandey, Darrel Jay Akiatan, James Hebditch, Celia David, Dharini Sapkal, Shaina Mae Monungolh, Varun Sane, Mark Lloyd Pielago, Miguel Albero, Jacquilyn Laude, Márcia dos Santos, Zeba Vohra, Kaiyu Wang, Allien Mae Gogo, Emil Kind, Alvin Josh Mandahay, Chereb Martinez, John David Asis, Chitra Nair, Dhwani Patel, Marchan Manaytay, Imaan F. M. Tamimi, Clyde Angelo Lim, Philip Lenard Ampo, Michelle Darapan Pantujan, Alexandre Javier, Daril Bautista, Rashmita Rana, Jansen Seguido
Abstract
Abstract Connections between neurons can be mapped by acquiring and analysing electron microscopic brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative 1–6 , but nevertheless inadequate for understanding brain function more globally. Here we present a neuronal wiring diagram of a whole brain containing 5 × 10 7 chemical synapses 7 between 139,255 neurons reconstructed from an adult female Drosophila melanogaster 8,9 . The resource also incorporates annotations of cell classes and types, nerves, hemilineages and predictions of neurotransmitter identities 10–12 . Data products are available for download, programmatic access and interactive browsing and have been made interoperable with other fly data resources. We derive a projectome—a map of projections between regions—from the connectome and report on tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine and descending neurons) across both hemispheres and between the central brain and the optic lobes. Tracing from a subset of photoreceptors to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviours. The technologies and open ecosystem reported here set the stage for future large-scale connectome projects in other species.