Proteomics and metabolomics analysis reveal potential mechanism of extended-spectrum β-lactamase production in <i>Escherichia coli</i>
He Ma, Bingjie Lai, Yufen Jin, Chang Fu Tian, Jiaying Liu, Ke Wang
Abstract
. The correlation analysis of metabolomics and proteomics data established a correlation between DEPs and differential metabolites in the purine metabolism pathway. Moreover, three metabolite candidates in the purine metabolism pathway were validated by the UPLC-MRM-MS (ultra-performance liquid chromatography multiple reaction monitoring mass spectrometry) method. Our data suggest that these DEPs and differential metabolites may play important roles in the antibiotic resistance of ESBL-EC. Our study can provide scientific data for the mechanism study of antibiotic resistance of ESBL-EC at the metabolite and protein levels and targeting modulators to these pathways may be effective for treatment of ESBL-EC strains.