Maternal high-fat diet modifies epigenetic marks H3K27me3 and H3K27ac in bone to regulate offspring osteoblastogenesis in mice
Jin‐Ran Chen, Perry C. Caviness, Haijun Zhao, Beau Belcher, Umesh D. Wankhade, Kartik Shankar, Michael L. Blackburn, Oxana P. Lazarenko
Abstract
had significantly enriched peaks of H3K27ac in HFD samples compared to controls. Their increased gene expression and association with H3K27ac were also confirmed by real-time PCR and standard ChIP analysis. These findings indicate that chronic maternal HFD changes histone trimethylation and acetylation epigenetic marks to regulate expression of genes controlling osteoblastogenesis.
Topics & Concepts
EpigeneticsBiologyH3K4me3DNA methylationOffspringHistoneEpigenomeCalvariaGeneticsOsteoblastEndocrinologyGene expressionCell biologyMolecular biologyPromoterGenePregnancyIn vitroEpigenetics and DNA MethylationBirth, Development, and HealthRNA modifications and cancer