A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H<sub>2</sub>O<sub>2</sub>
Jialing Zhou, Ruoxi Zhao, Yaqian Du, Shikai Liu, Wenting Li, Shili Gai, Fei He, Lili Feng, Piaoping Yang
Abstract
Abstract Hydrogen peroxide is an important reactive oxygen species (ROS) that acts as a significant marker of oxidative stress in certain diseases. Owing to the autofluorescence of biological samples, H 2 O 2 monitoring remains challenging. Here, Si‐CdTe quantum dots (QDs) are prepared as novel ratiometric fluorescent probes prepared for sensitive detection of H 2 O 2 and glucose, the low detection limit of H 2 O 2 and glucose is 79 and 140 n m , respectively. In this article, CdTe QDs are combined with Si QDs to reduce the cytotoxicity of CdTe. The survival rate of HeLa cells remains at 80% after incubation with the Si‐CdTe QDs at a concentration of 500 µg mL −1 for 24 h. Fluorescence extinguishes based on 488 and 514 nm channels are used as internal references to avoid environmental interference and demonstrate selective H 2 O 2 biosensing. In addition, the probe has an excellent colocalization effect in the lysosome under the 514 nm channel, with a maximum Pearson coefficient of 0.96. The advantages of easy preparation, low toxicity, high resistance to photobleaching, and high specificity of the Si‐CdTe QDs probes provide a new and convenient means to monitor and detect changes in the endogenous intracellular H 2 O 2 concentration and to select 514 nm lysosomal colocalization dyes.