Adaptive optics for optical microscopy [Invited]
Qinrong Zhang, Qi Hu, Caroline Berlage, Peter Kner, Benjamin Judkewitz, Martin J. Booth, Na Ji
Abstract
Optical microscopy is widely used to visualize fine structures. When applied to bioimaging, its performance is often degraded by sample-induced aberrations. In recent years, adaptive optics (AO), originally developed to correct for atmosphere-associated aberrations, has been applied to a wide range of microscopy modalities, enabling high- or super-resolution imaging of biological structure and function in complex tissues. Here, we review classic and recently developed AO techniques and their applications in optical microscopy.
Topics & Concepts
MicroscopyOpticsAdaptive opticsLight sheet fluorescence microscopyOptical microscopeSuper-resolution microscopyConfocal microscopyOptical imagingMaterials scienceComputer scienceScanning confocal electron microscopyPhysicsScanning electron microscopeAdvanced Fluorescence Microscopy TechniquesPhotoacoustic and Ultrasonic ImagingOptical Coherence Tomography Applications