An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
Ying Tang, Yiqin Wang, Yuchang Li, Huai Zhao, Sen Zhang, Ying Zhang, Jing Li, Yuehong Chen, Xiaoyan Wu, Cheng‐Feng Qin, Tao Jiang, Xiaoping Kang
Abstract
• I-RPA was developed by combining sample treatment and RPA detection in a single sealed cartridge. • No cross reaction was found in respiratory tract-associated viruses. • The low limit of detection was 35 copies/reaction. • This I-RPA assay is suitable for detection and monitoring of SARS-CoV-2 in the field.
Topics & Concepts
Detection limitVirologyNucleic acidSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Recombinant DNARecombinase Polymerase AmplificationCoronavirus disease 2019 (COVID-19)Molecular biologyPolymerase chain reactionChemistryBiologyChromatographyGeneMedicineBiochemistryInfectious disease (medical specialty)PathologyDiseaseSARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques