Photoredox‐Mediated Hydrogen Isotope Exchange Reactions of Amino‐Acids, Peptides, and Peptide‐Derived Drugs
Fabien Legros, Patricia Fernandez‐Rodriguez, Anurag Mishra, Remo Weck, Armin Bauer, Martin Sandvoß, Sven Ruf, Marı́a Méndez, Helena Mora‐Radó, Nils Rackelmann, Christoph Pöverlein, Volker Derdau
Abstract
Abstract Hydrogen isotopically labelled compounds are essential diagnostic tools in drug research and development, as they provide vital information about the biological metabolism of drug candidates and their metabolites. Herein we report a photoredox‐initiated hydrogen atom transfer (HAT) protocol which efficiently and selectively introduces deuterium or tritium at C(sp 3 )−H bonds, utilizing heavy water (D 2 O or T 2 O) as the hydrogen isotope source, and a guanidine base. This protocol has been successfully applied to the incorporation of deuterium in several amino acids (lysine, glycine and proline) and small peptides. Finally, the method has been applied to tritium, because tritium‐labelled peptides are essential for application in biological experiments, such as ligand‐binding assays, or absorption, distribution, metabolism, and excretion (ADME) studies.