Characterization of Silver Nanoparticles Synthesized using Chemical Method and its Antibacterial Property
Veeraswamy Thiruvengadam, Ankur Bansod, N Murugan, D Natarajan, L Ruddaraju, P Pallela, S Pammi, V Padavala, V Kolapalli, S Nakamura, M Sato, Y Sato, N Ando, T Takayama, M Fujita, M Ishihara, M Ahmad, H Badi'ah, F Seedeh, G Supriyanto, A Zaidan, J Song, B Kim, D Malina, A Sobczak-Kupiec, Z Wzorek, Z Kowalski, R Hamouda, M Hussein, R Abo-Elmagd, S Bawazir, S Bahlol, F Foda, J Ma, H Han, A Alshareef, K Laird, R Cross, K Mavani, M Shah, K Song, S Lee, T Park, B Lee, M Gao, L Sun, Z Wang, Y Zhao, S Ahmed, M Saifullah; Ahmad, B Swami, S Ikram, K Jyoti, M Baunthiyal, A Singh, A Mostafa, A Al-Askar, K Almaary, T Dawoud, E Sholkamy, M Bakri, H Ibrahim, N Vigneshwaran, N Ashtaputre, P Varadarajan, R Nachane, K Paralikar, R Balasubramanya, L Dorobantu, G Goss, R Burrell, A Kosmala, R Wright, Q Zhang, P Kirby, F Gulbagca, S Ozdemir, M Gulcan, F Sen, A Raza, Z Kanwal, A Rauf, N Sabri, S Riaz, S Naseem, V Kumar, R Wadhwa, N Kumar, P Maurya, X Vu, T Duong, T Pham, D Trinh, X Nguyen, V.-S Dang
Abstract
Metal nanoparticles are gaining importance nowadays in nanoscience. The nanoparticle had better physical and chemical properties compared with solid particles due to their large surface area. The silver nanoparticles are employed mostly in medical and electrical applications having outstanding conductivity and antimicrobial activity. In the present investigation, NaBH4 and ethanol were used as a reductant and stabilizer agent from silver nitrate salt as a precursor. The silver nanoparticles obtained were characterized using Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM) to determine their morphology and size. In XRD analysis, the average particle size was found to be 18.31 nm. The TEM analysis shows crystalline morphology with a face-centered cubic structure. The antibacterial activity was tested against two bacterial cultures, namely Bacillus subtilis and Pseudomonas aeruginosa. The inhibition zones of 19mm and 17mm were observed against Bacillus subtilis and Pseudomonas aeruginosa, respectively.