Directed reconstruction of a novel ancestral alcohol dehydrogenase featuring shifted pH-profile, enhanced thermostability and expanded substrate spectrum
Xiaoyu Chen, Zhe Dou, Tianwei Luo, Zewen Sun, Hongmin Ma, Guochao Xu, Ye Ni
Topics & Concepts
ThermostabilityAlcohol dehydrogenaseSubstrate (aquarium)Alcohol oxidoreductaseSubstrate specificityChemistryAlcoholBiochemistryDirected evolutionEnzymeBiologyNAD+ kinaseGeneMutantEcologyEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionBiofuel production and bioconversion