A Modular Approach for the Synthesis of Diverse Heterobifunctional Cyanine Dyes
Corina Maller, Franziska Schedel, Maja Köhn
Abstract
Herein, we present a straightforward synthetic route for the design and synthesis of diverse heterobifunctional cyanine 5 dyes. We optimized the workup by harnessing the pH- and functional group-dependent solubility of the asymmetric cyanine 5 dyes. Therefore, purification through chromatography is deferred until the last synthesis step. Demonstrating successful large-scale synthesis, our modular approach prevents functional group degradation by introducing them in the last synthesis step. These modifiable heterobifunctional dyes offer significant utility in advancing biological studies.
Topics & Concepts
CyanineChemistryModular designCombinatorial chemistryFunctional groupSolubilityChemical synthesisOrganic chemistryComputer scienceBiochemistryIn vitroProgramming languagePhysicsPolymerQuantum mechanicsFluorescenceClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniquesChemical Synthesis and Analysis