Toward Single Bacterium Proteomics
Ákos Végvári, Xuepei Zhang, Roman A. Zubarev
Abstract
High Resolution Image Download MS PowerPoint Slide Bacteria are orders of magnitude smaller than mammalian cells, and while single cell proteomics (SCP) currently detects and quantifies several thousands of proteins per mammalian cell, it is not clear whether conventional SCP methods will be suitable for bacteria. Here we report on the first successful attempt to detect proteins from individual Escherichia coli bacteria, with validation of our findings by comparison with two bacteria samples and bulk proteomics data. Data are available via ProteomeXchange with the identifier PXD043473.
Topics & Concepts
ProteomicsBacteriaEscherichia coliChemistryComputational biologyQuantitative proteomicsIdentifierBiochemistryBiologyGeneticsComputer scienceGeneProgramming languageBacteriophages and microbial interactionsGenomics and Phylogenetic StudiesRNA and protein synthesis mechanisms