A Defucosylated Mouse Anti-CD10 Monoclonal Antibody (31-mG <sub>2a</sub> -f) Exerts Antitumor Activity in a Mouse Xenograft Model of CD10-Overexpressed Tumors
Hiroki Kawabata, Hiroyuki Suzuki, Tomokazu Ohishi, Manabu Kawada, Mika K. Kaneko, Yukinari Kato
Abstract
CD10 is a glycosylated transmembrane protein and is known as a membrane endopeptidase. It is expressed on predifferentiated lymphocyte progenitor, epithelial, stromal, and tumor cells. Therefore, antibodies against CD10 are used for diagnosing follicular lymphoma and solid tumors, including renal carcinomas. In this study, we developed an anti-human CD10 monoclonal antibody, clone C 10 Mab-31 (IgG 1 , kappa), which detects CD10 by flow cytometry and shows high affinity for CD10-overexpressed CHO-K1 (CHO/CD10) cells. Furthermore, the defucosylated mouse IgG 2a version of C 10 Mab-31 (31-mG 2a -f) exhibits antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity, and antitumor activities in mouse xenografts of CHO/CD10 cells. These results indicate that 31-mG 2a -f exerts antitumor effects against CD10-expressing tumors and could be valuable as part of an antibody treatment regimen for them.