Blue Light Signaling Regulates Escherichia coli W1688 Biofilm Formation and <scp>l</scp> -Threonine Production
Wenjun Sun, Shuqi Shi, Jiao Chen, Wei Zhao, Tianpeng Chen, Guoxiong Li, Kaijie Zhang, Bin Yu, Dong Liu, Yong Chen, Hanjie Ying, Pingkai Ouyang
Abstract
We confirmed the mechanism underlying the inhibitory effects of blue light signaling on E. coli biofilm formation and constructed a strain lacking a blue light-sensing protein; this mitigated the aforementioned effects of blue light signaling and ensured normal fermentation performance. Furthermore, this study elucidated that the blue light signaling-induced optogenetic system Magnet effectively regulates E. coli biofilm formation and contributes to l-threonine production. This study not only enriches the mechanism of blue light signaling to regulate E. coli biofilm formation but also provides a theoretical basis and feasibility reference for the application of optogenetics technology in biofilm-based immobilized fermentation systems.