Activation of TGR5 restores AQP2 expression via the HIF pathway in renal ischemia-reperfusion injury
Mengke Han, Suchun Li, Haixia Xie, Qiaojuan Liu, Ani Wang, Shan Hu, Xiaoduo Zhao, Yonglun Kong, Weidong Wang, Chunling Li
Abstract
Stimulation of the membrane G protein-coupled bile acid receptor TGR5 by lithocholic acid (LCA) reduced polyuria in rats with renal ischemia-reperfusion (I/R) injury. LCA increased abundance of aquaporin-2 (AQP2) protein and upregulated hypoxia-inducible factor (HIF)-1α protein expression in association with decreased NF-κB p65 and IL-1β. After I/R, mice with tgr5 gene deficiency exhibited more severe decreases in AQP2 and HIF-1α protein abundance and inflammatory responses. TGR5 activation exhibits a protective role in acute renal injury induced by I/R.
Topics & Concepts
G protein-coupled bile acid receptorAquaporin 2KidneyEndocrinologyDownregulation and upregulationInternal medicineLithocholic acidReceptorHypoxia-inducible factorsReperfusion injuryChemistryBiologyBile acidMedicineIschemiaBiochemistryGeneMechanical engineeringEngineeringWater channelInletIon Transport and Channel RegulationDrug Transport and Resistance MechanismsAcute Kidney Injury Research