New Antibacterial Chloro-Containing Polyketides from the Alga-Derived Fungus Asteromyces cruciatus KMM 4696
Olesya I. Zhuravleva, G. K. Oleinikova, Alexandr S. Antonov, Н. Н. Киричук, Dmitry N. Pelageev, Anton B. Rasin, Alexander S. Menshov, Roman S. Popov, Natalya Yu. Kim, Ekaterina A. Chingizova, Artur R. Chingizov, Olga Olegovna Volchkova, Gunhild von Amsberg, Sergey A. Dyshlovoy, Ekaterina A. Yurchenko, Irina V. Guzhova, Anton N. Yurchenko
Abstract
Six new polyketides acrucipentyns A–F (1–6) were isolated from the alga-derived fungus Asteromyces cruciatus KMM 4696. Their structures were established based on spectroscopic methods. The absolute configurations of acrucipentyn A was assigned by the modified Mosher’s method and ROESY data analysis. Acrucipentyns A–E were identified to be the very first examples of chlorine-containing asperpentyn-like compounds. The cytotoxic and antimicrobial activities of the isolated compounds were examined. Acrucipentyns A–F were found as antimicrobial agents, which inhibited sortase A enzyme activity, bacterial growth and biofilm formation of Staphylococcus aureus and decreased LDH release from human keratinocytes HaCaT in S. aureus skin infection in an in vitro model.