Aptasensing of ciprofloxacin residue using graphene oxide modified with gold nanoparticles and branched polyethyleneimine
Mansour Mahmoudpour, Jafar Ezzati Nazhad Dolatabadi, Mohammad Hasanzadeh, Aziz Homayouni Rad, Mohammadali Torbati, Farzad Seidi
Abstract
cyclic voltammetry techniques. The construction and morphology of the GO-PEI-AuNPs composite were evaluated by UV-Vis spectroscopy, transmission electron microscopy, and field emission scanning electron microscopy. Under optimal conditions, the suggested scaffold can offer an acceptable linear range of 0.001 to 100 μM and a low limit of quantification of 0.001 μM for selective and sensitive monitoring of CFX in real samples. The effectiveness of the apta-assay was confirmed by detection of CFX in pasteurized and local milk samples for which suitable analytical results were achieved. It is expected that the developed substrate can be facilely extended to other aptamer-based multiplex screening platforms in actual food and environmental samples.