<i>In Vitro</i> Elimination of EL4 Cancer Cells From Spermatogonia Stem Cells by Mirna-143- and 206-Loaded Folic Acid-Conjugated Plga Nanoparticles
Azar Shams, Ronak Shabani, Hamid Reza Asgari, Mahdi Karimi, Mohammad Najafi, Mohammad Asghari Jafarabadi, Seyed Mohsen Razavi, Seyed Rouhollah Miri, Mehdi Abbasi, Amirhossein Mohammadi, Morteza Koruji
Abstract
Aim: MiRNA's-143 and -206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- and 206-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. Materials & methods: The therapy was conducted in six groups: treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. Results: In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. Conclusion: MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable.