Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans
Michael L. Nonet
Abstract
Recombination-mediated cassette exchange (RMCE) is a recently developed alternative method for creating single copy transgenes using recombination rather than repair of double stranded breaks as the mechanism for driving integration into the genome. Two alternative methods for performing RMCE have been developed; a two-component approach using an unlinked source of FLP recombinase, and a one-component approach using a FLP expression cassette within the landing site. Here, I describe new landing sites for performing both types of RMCE. The new landing sites are located within 50 bp of well-vetted MosSCI insertion sites on Chr II and Chr IV.
Topics & Concepts
RecombinaseSite-specific recombinationRecombinationFLP-FRT recombinationBiologyComputational biologyGeneticsExpression cassetteGeneVector (molecular biology)Genetic recombinationRecombinant DNACRISPR and Genetic EngineeringGenetics, Aging, and Longevity in Model OrganismsPhotosynthetic Processes and Mechanisms