Supercritical CO2 Extraction and Identification of Ginsenosides in Russian and North Korean Ginseng by HPLC with Tandem Mass Spectrometry
Mayya P. Razgonova, Alexander M. Zakharenko, Tai-Sun Shin, Gyuhwa Chung, Kirill S. Golokhvast
Abstract
Ginseng roots, Panax ginseng C.A. Meyer, obtained from cultivated ginseng grown in the Kaesong province (North Korea) and Primorye (Russia) were extracted using the supercritical CO2 extraction method. The extracts were subsequently analyzed by high-performance liquid chromatography with tandem mass spectrometry identification. The results showed the spectral peaks of typical ginsenosides with some other minor groups, and major differences were observed between the spectra of the two ginseng samples. The use of a pressure of 400 bar and higher allowed an increase in the yield of ginsenosides in comparison with similar previous studies
Topics & Concepts
GinsengChromatographyGinsenosideSupercritical fluidChemistryExtraction (chemistry)Mass spectrometryTandem mass spectrometryAraliaceaeDammaraneHigh-performance liquid chromatographyYield (engineering)Materials scienceMedicineTriterpeneOrganic chemistryMetallurgyPathologyAlternative medicineGinseng Biological Effects and ApplicationsTraditional Chinese Medicine AnalysisPharmacological Effects of Natural Compounds