Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era
Rosiris León‐Rivera, Mike Veenstra, Maribel Donoso, Elizabeth Tell, Eliseo A. Eugenín, Susan Morgello, Joan W. Berman
Abstract
We characterized mechanisms of CNS viral reservoir establishment/replenishment using peripheral blood mononuclear cells (PBMC) of PLWH on cART and propose therapeutic targets to reduce/block selective entry of cells harboring HIV (HIV + ) into the CNS. Using DNA/RNAscope, we show that CD14 + CD16 + monocytes with integrated HIV, transcriptionally active, and/or with active viral replication from PBMC of PLWH prescribed cART and virally suppressed, selectively transmigrate across a human BBB model.
Topics & Concepts
CD14CartPeripheral blood mononuclear cellVirologyImmunologyCentral nervous systemBiologyVirusCD16Viral replicationMonocyteImmune systemMedicineIn vitroCD3CD8EngineeringNeuroscienceBiochemistryMechanical engineeringHIV Research and TreatmentCytomegalovirus and herpesvirus researchHIV-related health complications and treatments