Adsorption of Horseradish Peroxidase on Metallic Nanoparticles: Effects on Reactive Oxygen Species Detection Using 2′,7′-Dichlorofluorescin Diacetate
Amanda Kessler, Jonas Hedberg, Sarah McCarrick, Hanna L. Karlsson, Eva Blomberg, Inger Odnevall Wallinder
Abstract
to reach the active site of HRP. This study further emphasizes the influence of HRP on the background kinetics, its solution dependence, and effects on measured ROS signals. Different ways of correcting for the background are highlighted, as this can result in different interpretations of generated results. The results show that adsorption of HRP onto the metal NPs influenced the extent of metal release and may, depending on the investigated system, result in either under- or overestimated ROS signals if used together with the DCFH assay. HRP should hence be used with caution when measuring ROS in the presence of reactive metallic NPs.
Topics & Concepts
ChemistryHorseradish peroxidaseAdsorptionNuclear chemistryMetalReactive oxygen speciesNanoparticleDichlorofluoresceinHydrogen peroxidePhosphateMetal ions in aqueous solutionInorganic chemistryOrganic chemistryChemical engineeringBiochemistryEnzymeEngineeringNanoparticles: synthesis and applicationsHeavy Metal Exposure and ToxicityElectrochemical Analysis and Applications